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HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeAfefab3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernatantfefabs 48 h later。

Briefly, FeFAB cells are the modified CRFK cells that express β-galactosidase under the control of the FFV LTR, which is trans-activated by the FFV Tas protein in relation to。

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